Plasmid Preparation:Article Title: Microcircuit mechanisms for the generation of sharp-wave ripples in the basolateral amygdala: A role for chandelier interneurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Ankyrin G Santa Cruz Cat#: sc28561; RRID:AB_633909 Mouse anti-parvalbumin Sigma-Aldrich Cat#: P3088; RRID:AB_477329 Goat anti-mouse, Alexa 405 Life Technologies Cat#:A31553;RRID:AB_221604 Goat anti-rabbit, Alexa 647 Invitrogen Cat#:A21245;RRID:AB_2535813 Alexa 555-Streptavidin Invitrogen Cat# S32355;RRID:AB_2571525 Alexa 647-Streptavidin Invitrogen Cat#: S21374;RRID:AB_2336066 Bacterial and virus strains pAAV-EF1a-DIO-hM4D(Gi)-mCherry Addgene Plasmid#: 50461;RRID:Addgene_50461 Chemicals, peptides, and recombinant proteins Cal520 AM Abcam CAT#: 21130 Bicuculline Tocris CAS# 485-49-4 Picrotoxin Sigma-Aldrich CAS#: 124-87-8 Gabazine Tocris CAS#: 104104-50-9 NBQX Tocris CAS# 118876-58-7 CNQX Tocris CAS#: 115066-14-3 Clozapine-N-oxide AK Scientific CAS#: 34233-69-7 Biocytin Sigma-Aldrich CAS#: 576-19-2 Linopiridine Tocris CAS#: 113168-57-3 Experimental models: organisms/strains Rat: Sprague Dawley Animal facility, Queensland Brain Institute, The University of Queensland Rat: Wistar Animal facility, Queensland Brain Institute, The University of Queensland Mice: C57BL/6NCrj xCBA/JNCrj) F1 Animal facility, Queensland Brain Institute, The University of Queensland Mice: B6;129P2-Pvalbtm1(cre)Arbr/J Animal facility, Queensland Brain Institute, The University of Queensland Mice: WT (C57BL/6 and BALB/c background) Animal facility, Queensland Brain Institute, The University of Queensland Software and algorithms Axograph X Axograph Scientific RRID:SCR_014284 Neurolucida MicroBrightField RRID: SCR_001775 NEURON https://neuron.yale.edu/neuron/ RRID: SCR_005393 MATLAB (R_2018a) Mathworks RRID: SCR_001622 Fiji(ImageJ) https://imagej.nih.gov/ij/ RRID: SCR_002285 GraphPad Prism (Version 7 and 8) GraphPad RRID: SCR_002798 Other Vibrotome Leica VT1000S Patch clamp amplifier Molecular Devices Multiclamp 700A and 700B ITC-16 A/D converter InstruTECH HumBug Digitimer Cell Reports 35, 109106, May 11, 2021 e1
Recombinant:Article Title: Microcircuit mechanisms for the generation of sharp-wave ripples in the basolateral amygdala: A role for chandelier interneurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Ankyrin G Santa Cruz Cat#: sc28561; RRID:AB_633909 Mouse anti-parvalbumin Sigma-Aldrich Cat#: P3088; RRID:AB_477329 Goat anti-mouse, Alexa 405 Life Technologies Cat#:A31553;RRID:AB_221604 Goat anti-rabbit, Alexa 647 Invitrogen Cat#:A21245;RRID:AB_2535813 Alexa 555-Streptavidin Invitrogen Cat# S32355;RRID:AB_2571525 Alexa 647-Streptavidin Invitrogen Cat#: S21374;RRID:AB_2336066 Bacterial and virus strains pAAV-EF1a-DIO-hM4D(Gi)-mCherry Addgene Plasmid#: 50461;RRID:Addgene_50461 Chemicals, peptides, and recombinant proteins Cal520 AM Abcam CAT#: 21130 Bicuculline Tocris CAS# 485-49-4 Picrotoxin Sigma-Aldrich CAS#: 124-87-8 Gabazine Tocris CAS#: 104104-50-9 NBQX Tocris CAS# 118876-58-7 CNQX Tocris CAS#: 115066-14-3 Clozapine-N-oxide AK Scientific CAS#: 34233-69-7 Biocytin Sigma-Aldrich CAS#: 576-19-2 Linopiridine Tocris CAS#: 113168-57-3 Experimental models: organisms/strains Rat: Sprague Dawley Animal facility, Queensland Brain Institute, The University of Queensland Rat: Wistar Animal facility, Queensland Brain Institute, The University of Queensland Mice: C57BL/6NCrj xCBA/JNCrj) F1 Animal facility, Queensland Brain Institute, The University of Queensland Mice: B6;129P2-Pvalbtm1(cre)Arbr/J Animal facility, Queensland Brain Institute, The University of Queensland Mice: WT (C57BL/6 and BALB/c background) Animal facility, Queensland Brain Institute, The University of Queensland Software and algorithms Axograph X Axograph Scientific RRID:SCR_014284 Neurolucida MicroBrightField RRID: SCR_001775 NEURON https://neuron.yale.edu/neuron/ RRID: SCR_005393 MATLAB (R_2018a) Mathworks RRID: SCR_001622 Fiji(ImageJ) https://imagej.nih.gov/ij/ RRID: SCR_002285 GraphPad Prism (Version 7 and 8) GraphPad RRID: SCR_002798 Other Vibrotome Leica VT1000S Patch clamp amplifier Molecular Devices Multiclamp 700A and 700B ITC-16 A/D converter InstruTECH HumBug Digitimer Cell Reports 35, 109106, May 11, 2021 e1
Mouse Assay:Article Title: Microcircuit mechanisms for the generation of sharp-wave ripples in the basolateral amygdala: A role for chandelier interneurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Ankyrin G Santa Cruz Cat#: sc28561; RRID:AB_633909 Mouse anti-parvalbumin Sigma-Aldrich Cat#: P3088; RRID:AB_477329 Goat anti-mouse, Alexa 405 Life Technologies Cat#:A31553;RRID:AB_221604 Goat anti-rabbit, Alexa 647 Invitrogen Cat#:A21245;RRID:AB_2535813 Alexa 555-Streptavidin Invitrogen Cat# S32355;RRID:AB_2571525 Alexa 647-Streptavidin Invitrogen Cat#: S21374;RRID:AB_2336066 Bacterial and virus strains pAAV-EF1a-DIO-hM4D(Gi)-mCherry Addgene Plasmid#: 50461;RRID:Addgene_50461 Chemicals, peptides, and recombinant proteins Cal520 AM Abcam CAT#: 21130 Bicuculline Tocris CAS# 485-49-4 Picrotoxin Sigma-Aldrich CAS#: 124-87-8 Gabazine Tocris CAS#: 104104-50-9 NBQX Tocris CAS# 118876-58-7 CNQX Tocris CAS#: 115066-14-3 Clozapine-N-oxide AK Scientific CAS#: 34233-69-7 Biocytin Sigma-Aldrich CAS#: 576-19-2 Linopiridine Tocris CAS#: 113168-57-3 Experimental models: organisms/strains Rat: Sprague Dawley Animal facility, Queensland Brain Institute, The University of Queensland Rat: Wistar Animal facility, Queensland Brain Institute, The University of Queensland Mice: C57BL/6NCrj xCBA/JNCrj) F1 Animal facility, Queensland Brain Institute, The University of Queensland Mice: B6;129P2-Pvalbtm1(cre)Arbr/J Animal facility, Queensland Brain Institute, The University of Queensland Mice: WT (C57BL/6 and BALB/c background) Animal facility, Queensland Brain Institute, The University of Queensland Software and algorithms Axograph X Axograph Scientific RRID:SCR_014284 Neurolucida MicroBrightField RRID: SCR_001775 NEURON https://neuron.yale.edu/neuron/ RRID: SCR_005393 MATLAB (R_2018a) Mathworks RRID: SCR_001622 Fiji(ImageJ) https://imagej.nih.gov/ij/ RRID: SCR_002285 GraphPad Prism (Version 7 and 8) GraphPad RRID: SCR_002798 Other Vibrotome Leica VT1000S Patch clamp amplifier Molecular Devices Multiclamp 700A and 700B ITC-16 A/D converter InstruTECH HumBug Digitimer Cell Reports 35, 109106, May 11, 2021 e1
Software:Article Title: Microcircuit mechanisms for the generation of sharp-wave ripples in the basolateral amygdala: A role for chandelier interneurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Ankyrin G Santa Cruz Cat#: sc28561; RRID:AB_633909 Mouse anti-parvalbumin Sigma-Aldrich Cat#: P3088; RRID:AB_477329 Goat anti-mouse, Alexa 405 Life Technologies Cat#:A31553;RRID:AB_221604 Goat anti-rabbit, Alexa 647 Invitrogen Cat#:A21245;RRID:AB_2535813 Alexa 555-Streptavidin Invitrogen Cat# S32355;RRID:AB_2571525 Alexa 647-Streptavidin Invitrogen Cat#: S21374;RRID:AB_2336066 Bacterial and virus strains pAAV-EF1a-DIO-hM4D(Gi)-mCherry Addgene Plasmid#: 50461;RRID:Addgene_50461 Chemicals, peptides, and recombinant proteins Cal520 AM Abcam CAT#: 21130 Bicuculline Tocris CAS# 485-49-4 Picrotoxin Sigma-Aldrich CAS#: 124-87-8 Gabazine Tocris CAS#: 104104-50-9 NBQX Tocris CAS# 118876-58-7 CNQX Tocris CAS#: 115066-14-3 Clozapine-N-oxide AK Scientific CAS#: 34233-69-7 Biocytin Sigma-Aldrich CAS#: 576-19-2 Linopiridine Tocris CAS#: 113168-57-3 Experimental models: organisms/strains Rat: Sprague Dawley Animal facility, Queensland Brain Institute, The University of Queensland Rat: Wistar Animal facility, Queensland Brain Institute, The University of Queensland Mice: C57BL/6NCrj xCBA/JNCrj) F1 Animal facility, Queensland Brain Institute, The University of Queensland Mice: B6;129P2-Pvalbtm1(cre)Arbr/J Animal facility, Queensland Brain Institute, The University of Queensland Mice: WT (C57BL/6 and BALB/c background) Animal facility, Queensland Brain Institute, The University of Queensland Software and algorithms Axograph X Axograph Scientific RRID:SCR_014284 Neurolucida MicroBrightField RRID: SCR_001775 NEURON https://neuron.yale.edu/neuron/ RRID: SCR_005393 MATLAB (R_2018a) Mathworks RRID: SCR_001622 Fiji(ImageJ) https://imagej.nih.gov/ij/ RRID: SCR_002285 GraphPad Prism (Version 7 and 8) GraphPad RRID: SCR_002798 Other Vibrotome Leica VT1000S Patch clamp amplifier Molecular Devices Multiclamp 700A and 700B ITC-16 A/D converter InstruTECH HumBug Digitimer Cell Reports 35, 109106, May 11, 2021 e1
Patch Clamp:Article Title: Microcircuit mechanisms for the generation of sharp-wave ripples in the basolateral amygdala: A role for chandelier interneurons.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Ankyrin G Santa Cruz Cat#: sc28561; RRID:AB_633909 Mouse anti-parvalbumin Sigma-Aldrich Cat#: P3088; RRID:AB_477329 Goat anti-mouse, Alexa 405 Life Technologies Cat#:A31553;RRID:AB_221604 Goat anti-rabbit, Alexa 647 Invitrogen Cat#:A21245;RRID:AB_2535813 Alexa 555-Streptavidin Invitrogen Cat# S32355;RRID:AB_2571525 Alexa 647-Streptavidin Invitrogen Cat#: S21374;RRID:AB_2336066 Bacterial and virus strains pAAV-EF1a-DIO-hM4D(Gi)-mCherry Addgene Plasmid#: 50461;RRID:Addgene_50461 Chemicals, peptides, and recombinant proteins Cal520 AM Abcam CAT#: 21130 Bicuculline Tocris CAS# 485-49-4 Picrotoxin Sigma-Aldrich CAS#: 124-87-8 Gabazine Tocris CAS#: 104104-50-9 NBQX Tocris CAS# 118876-58-7 CNQX Tocris CAS#: 115066-14-3 Clozapine-N-oxide AK Scientific CAS#: 34233-69-7 Biocytin Sigma-Aldrich CAS#: 576-19-2 Linopiridine Tocris CAS#: 113168-57-3 Experimental models: organisms/strains Rat: Sprague Dawley Animal facility, Queensland Brain Institute, The University of Queensland Rat: Wistar Animal facility, Queensland Brain Institute, The University of Queensland Mice: C57BL/6NCrj xCBA/JNCrj) F1 Animal facility, Queensland Brain Institute, The University of Queensland Mice: B6;129P2-Pvalbtm1(cre)Arbr/J Animal facility, Queensland Brain Institute, The University of Queensland Mice: WT (C57BL/6 and BALB/c background) Animal facility, Queensland Brain Institute, The University of Queensland Software and algorithms Axograph X Axograph Scientific RRID:SCR_014284 Neurolucida MicroBrightField RRID: SCR_001775 NEURON https://neuron.yale.edu/neuron/ RRID: SCR_005393 MATLAB (R_2018a) Mathworks RRID: SCR_001622 Fiji(ImageJ) https://imagej.nih.gov/ij/ RRID: SCR_002285 GraphPad Prism (Version 7 and 8) GraphPad RRID: SCR_002798 Other Vibrotome Leica VT1000S Patch clamp amplifier Molecular Devices Multiclamp 700A and 700B ITC-16 A/D converter InstruTECH HumBug Digitimer Cell Reports 35, 109106, May 11, 2021 e1
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